Hotspot

trnR-UCU

LSC · 12071-12142 · 72 bp

Back to Quillaja

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label trnR-UCU

Feature Metadata

label_name: trnR-UCU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	CATTTACCGGGGAAGCAGAA	50.0	50.0	58.0	57.9	770-799 bp	1.0	82.7
trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	959-988 bp	1.0	82.6
trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	962-991 bp	1.0	82.4
trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	GACCTGCGATTAATGTGGGT	50.0	50.0	58.0	58.0	1148-1177 bp	1.0	82.1
trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	GACCTGCGATTAATGTGGGT	55.0	50.0	58.1	58.0	1151-1180 bp	1.0	81.8