Hotspot

rps15

SSC · 128949-129221 · 273 bp

Back to Quillaja

Pi 0.00733

MarkerSeek score 42.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 2
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACTCCCTCAACCCGTTCTAA	AAGACAAAGAGATGCGCACA	50.0	45.0	58.0	58.1	1355-1366 bp	1.0	79.3
rps15_p2	AACACCATGCATTGATGGGT	AAGACAAAGAGATGCGCACA	45.0	45.0	58.1	58.1	813-824 bp	1.0	79.2
rps15_p3	TCTTGTAGCCGGGAGATTCA	AAGACAAAGAGATGCGCACA	50.0	45.0	58.1	58.1	759-770 bp	1.0	78.6
rps15_p4	AACTGTTCCGGTCCGTTTAC	AAGACAAAGAGATGCGCACA	50.0	45.0	58.1	58.1	1004-1015 bp	1.0	78.5
rps15_p5	GTTCCGGTCCGTTTACAGTT	AAGACAAAGAGATGCGCACA	50.0	45.0	58.1	58.1	1000-1011 bp	1.0	78.5