Hotspot

rpl32-trnL-UAG

SSC · 119132-119665 · 534 bp

Back to Quillaja

Pi 0.0207

MarkerSeek score 67.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 534
variable_sites: 11
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	663-666 bp	1.0	81.5
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	664-667 bp	1.0	81.5
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	664-667 bp	1.0	81.5
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	665-668 bp	1.0	81.4
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	673-676 bp	1.0	80.5