Hotspot

ndhF-rpl32

SSC · 117912-118963 · 1052 bp

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Pi 0.00583

MarkerSeek score 62.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1052
variable_sites: 6
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	TCAATGCTGCCCAATATCCC	45.0	50.0	58.0	58.0	1236-1239 bp	1.0	81.5
ndhF-rpl32_p2	ACATAGGAACCGGAAGTGGA	TCAATGCTGCCCAATATCCC	50.0	50.0	58.1	58.0	1198-1201 bp	1.0	81.5
ndhF-rpl32_p3	ACATAGGAACCGGAAGTGGA	CAATGCTGCCCAATATCCCT	50.0	50.0	58.1	58.0	1197-1200 bp	1.0	81.5
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	CAATGCTGCCCAATATCCCT	45.0	50.0	58.0	58.0	1235-1238 bp	1.0	81.4
ndhF-rpl32_p5	GGAACCGGAAGTGGAATGAA	TCAATGCTGCCCAATATCCC	50.0	50.0	57.8	58.0	1193-1196 bp	1.0	80.4