Hotspot

ycf1-ndhF

SSC · 115530-115670 · 141 bp

Back to Quillaja

Pi 0.0284

MarkerSeek score 60.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 4
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AGTTTTATTGGTTGGTTAGTTGA	GGTGGTCGAATATCTTCTTAT	30.4	38.1	54.4	52.6	303 bp	1.0	41.1
ycf1-ndhF_p2	ACAAGTAGTTTTATTGGTTGGT	GGTGGTCGAATATCTTCTTAT	31.8	38.1	53.8	52.6	309 bp	1.0	41.0
ycf1-ndhF_p3	AACAAGTAGTTTTATTGGTTGGT	GGTGGTCGAATATCTTCTTAT	30.4	38.1	54.6	52.6	310 bp	1.0	41.0
ycf1-ndhF_p4	ACAAGTAGTTTTATTGGTTGGTT	GGTGGTCGAATATCTTCTTAT	30.4	38.1	54.6	52.6	309 bp	1.0	41.0
ycf1-ndhF_p5	AACAAGTAGTTTTATTGGTTGGTT	GGTGGTCGAATATCTTCTTAT	29.2	38.1	55.4	52.6	310 bp	1.0	41.0