Hotspot

rps3-rpl22

LSC · 88570-88858 · 289 bp

Back to Quillaja

Pi 0.00403

MarkerSeek score 49.20

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 1
pi: 0.00403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAAGTCTGAAACCAAGCGGA	AATGGGTTTCTTCTGCAGCA	50.0	45.0	58.4	58.0	356-397 bp	1.0	81.0
rps3-rpl22_p2	AAGTCTGAAACCAAGCGGAT	AATGGGTTTCTTCTGCAGCA	45.0	45.0	57.4	58.0	355-396 bp	1.0	79.7
rps3-rpl22_p3	AGTCTGAAACCAAGCGGATT	AATGGGTTTCTTCTGCAGCA	45.0	45.0	57.4	58.0	354-395 bp	1.0	79.7
rps3-rpl22_p4	ACCAAGTCTGAAACCAAGCG	AATGGGTTTCTTCTGCAGCA	50.0	45.0	58.7	58.0	358-399 bp	1.0	78.7
rps3-rpl22_p5	CAAGTCTGAAACCAAGCGGA	ATGGGTTTCTTCTGCAGCAG	50.0	50.0	58.4	58.5	355-396 bp	1.0	77.3