Hotspot

petN-psbM

LSC · 31297-32519 · 1223 bp

Back to Quillaja

Pi 0.00329

MarkerSeek score 61.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 4
pi: 0.00329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1319-1324 bp	1.0	66.7
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1319-1324 bp	1.0	62.5
petN-psbM_p3	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTCT	47.6	42.9	58.0	57.6	1366-1371 bp	1.0	61.5
petN-psbM_p4	TGGGGAAGAAGTGGACTCTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.0	57.6	1318-1323 bp	1.0	61.4
petN-psbM_p5	GCTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	50.0	42.9	59.1	57.6	1369-1374 bp	1.0	60.7