Hotspot

atpH-atpI

LSC · 15853-16598 · 746 bp

Back to Quillaja

Pi 0.00536

MarkerSeek score 61.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 4
pi: 0.00536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACTCTCGCTGC	45.0	45.0	58.0	57.9	835-836 bp	1.0	79.6
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	ATTTTTGCAACTCTCGCTGC	55.0	45.0	58.0	57.9	920-921 bp	1.0	79.6
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	803-804 bp	1.0	78.7
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	888-889 bp	1.0	78.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACTCTCGCTGC	45.0	45.0	58.2	57.9	836-837 bp	1.0	78.5