Hotspot

trnS-GCU-trnG-UCC

LSC · 9652-10846 · 1195 bp

Back to Quillaja

Pi 0.00337
MarkerSeek score 61.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1195
variable_sites
4
pi
0.00337
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGATCAGTAGAACGAATCGCA 50.0 47.6 58.0 57.9 1269-1275 bp 1.0 72.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGATCAGTAGAACGAATCGCA 50.0 47.6 58.0 57.9 1268-1274 bp 1.0 72.1
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA GGATCAGTAGAACGAATCGCA 50.0 47.6 58.2 57.9 1323-1329 bp 1.0 70.9
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1258-1264 bp 1.0 70.9
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1257-1263 bp 1.0 70.9