Hotspot

ndhF

SSC · 116196-118475 · 2280 bp

Back to Quercus

Pi 0.0078

MarkerSeek score 48.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2280
variable_sites: 169
pi: 0.0078
unique_haplotype_count: 69
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGAGTACCGTCACCCATTG	AACACAAGGCTACAATCGCT	55.0	45.0	58.0	57.8	2811-2963 bp	0.956522	86.3
ndhF_p2	CAGAGTACCGTCACCCATTG	ACACAAGGCTACAATCGCTT	55.0	45.0	58.0	57.8	2810-2962 bp	0.956522	86.3
ndhF_p3	CAGAGTACCGTCACCCATTG	AAGAACTGGCGAATCCGAAA	55.0	45.0	58.0	57.8	2647-2791 bp	0.973913	86.0
ndhF_p4	ACGAAGGGGACTAAACAGGA	ACACAAGGCTACAATCGCTT	50.0	45.0	58.0	57.8	2715-2871 bp	0.956522	85.4
ndhF_p5	ACGAAGGGGACTAAACAGGA	AAGAACTGGCGAATCCGAAA	50.0	45.0	58.0	57.8	2554-2700 bp	0.973913	84.9