Hotspot

ccsA-ndhD

SSC · 121910-122231 · 322 bp

Back to Quercus

Pi 0.00824

MarkerSeek score 61.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 322
variable_sites: 37
pi: 0.00824
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATTGTAGCCTCCCTGGGAT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	58.1	57.5	461-483 bp	1.0	78.1
ccsA-ndhD_p2	ATTGTAGCCTCCCTGGGATT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	58.1	57.5	460-482 bp	1.0	78.1
ccsA-ndhD_p3	TTCTGCAATTGTAGCCTCCC	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.9	57.5	467-489 bp	1.0	77.7
ccsA-ndhD_p4	AGCCTCCCTGGGATTTCTTA	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.7	57.5	455-477 bp	1.0	76.6
ccsA-ndhD_p5	TAGCCTCCCTGGGATTTCTT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.7	57.5	456-478 bp	1.0	76.6