Hotspot

psaJ-rpl33

LSC · 72868-73361 · 494 bp

Back to Quercus

Pi 0.00758

MarkerSeek score 60.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 36
pi: 0.00758
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCGCTGATATTCCCCTT	TCGAACATCTTTCCCCTTGG	50.0	50.0	57.8	57.5	513-554 bp	1.0	84.2
psaJ-rpl33_p2	TTCCGGATGCGCTGATATTC	TCGAACATCTTTCCCCTTGG	50.0	50.0	58.2	57.5	518-559 bp	1.0	84.1
psaJ-rpl33_p3	TTTATTTCCGGATGCGCTGA	TCGAACATCTTTCCCCTTGG	45.0	50.0	58.0	57.5	523-564 bp	0.956522	83.4
psaJ-rpl33_p4	GTTTATTTCCGGATGCGCTG	TCGAACATCTTTCCCCTTGG	50.0	50.0	57.9	57.5	524-565 bp	0.956522	82.8
psaJ-rpl33_p5	TTTATTTCCGGATGCGCTGA	GCATCATTTCGAACAGCACA	45.0	45.0	58.0	57.4	570-611 bp	0.956522	82.0