Hotspot

psbE-petL

LSC · 70136-71478 · 1343 bp

Back to Quercus

Pi 0.00766

MarkerSeek score 67.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1343
variable_sites: 100
pi: 0.00766
unique_haplotype_count: 75
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGTTCAACCCG	50.0	47.6	57.8	58.0	1290-1478 bp	1.0	80.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAACCCGA	50.0	47.6	57.8	58.0	1289-1477 bp	1.0	80.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGTTCAACCCG	45.0	47.6	58.2	58.0	1376-1564 bp	1.0	79.7
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGTTCAACCCGA	45.0	47.6	58.2	58.0	1375-1563 bp	1.0	79.7
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGTCGTATCTTGTTCAACCCG	50.0	47.6	58.2	58.0	1381-1569 bp	1.0	79.6