Hotspot

rbcL-accD

LSC · 61794-62388 · 595 bp

Back to Quercus

Pi 0.0219

MarkerSeek score 54.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 40
pi: 0.0219
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACAATTTGGCGGAGGAACTT	GTTAACTCACTACCAGCCGG	45.0	55.0	58.0	58.0	1491-1533 bp	1.0	88.0
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1397-1439 bp	1.0	88.0
rbcL-accD_p3	CCGTACTACAATTTGGCGGA	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1498-1540 bp	1.0	87.9
rbcL-accD_p4	TCCGTACTACAATTTGGCGG	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1499-1541 bp	1.0	87.9
rbcL-accD_p5	GCCGTAGCTAATCGAGTAGC	GTTAACTCACTACCAGCCGG	55.0	55.0	58.0	58.0	1442-1484 bp	1.0	87.9