Hotspot

trnM-CAU-atpE

LSC · 57459-57658 · 200 bp

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Pi 0.0167
MarkerSeek score 57.30
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
200
variable_sites
15
pi
0.0167
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 288-299 bp 1.0 83.7
trnM-CAU-atpE_p2 ATCGATGTCATAGGCCCCAT CTCTCAGACGAGCTAGGACA 50.0 55.0 58.3 58.0 357-370 bp 0.965217 82.6
trnM-CAU-atpE_p3 CGATGTCATAGGCCCCATTT CTCTCAGACGAGCTAGGACA 50.0 55.0 57.7 58.0 355-368 bp 0.956522 82.6
trnM-CAU-atpE_p4 CGATGTCATAGGCCCCATTT TCTCAGACGAGCTAGGACAC 50.0 55.0 57.7 58.3 354-367 bp 0.956522 80.8
trnM-CAU-atpE_p5 TCGATGTCATAGGCCCCATT CTCTCAGACGAGCTAGGACA 50.0 55.0 58.6 58.0 356-369 bp 0.956522 80.2