Hotspot

rpoB-trnC-GCA

LSC · 29217-30484 · 1268 bp

Back to Quercus

Pi 0.00765

MarkerSeek score 62.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1268
variable_sites: 84
pi: 0.00765
unique_haplotype_count: 72
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCTACCCCCAAGC	TTAGGCGACACCCAGATTTG	55.0	50.0	57.9	57.9	1318-1498 bp	1.0	87.7
rpoB-trnC-GCA_p2	CCTCATTTCTACCCCCAAGC	TTGTTGATTAGGCGACACCC	55.0	50.0	57.9	58.2	1325-1505 bp	1.0	87.0
rpoB-trnC-GCA_p3	CCTCATTTCTACCCCCAAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1289-1469 bp	0.991304	85.5
rpoB-trnC-GCA_p4	TCCCTCATTTCTACCCCCAA	TTAGGCGACACCCAGATTTG	50.0	50.0	57.6	57.9	1320-1500 bp	1.0	85.2
rpoB-trnC-GCA_p5	TTCCCTCATTTCTACCCCCA	TTAGGCGACACCCAGATTTG	50.0	50.0	57.6	57.9	1321-1501 bp	1.0	85.2