Hotspot

rps16-trnQ-UUG

LSC · 6646-8247 · 1602 bp

Back to Quercus

Pi 0.00721
MarkerSeek score 57.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1602
variable_sites
129
pi
0.00721
unique_haplotype_count
72
primer_available
yes
alignment_reliability
0.94
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GACCATATCCCTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 1493-1732 bp 1.0 88.6
rps16-trnQ-UUG_p2 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1475-1714 bp 1.0 88.4
rps16-trnQ-UUG_p3 CCATATCCCTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1491-1730 bp 0.965217 87.2
rps16-trnQ-UUG_p4 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 1479-1718 bp 1.0 86.9
rps16-trnQ-UUG_p5 CGACCATATCCCTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.6 58.1 1494-1733 bp 1.0 86.5