Hotspot

trnH-GUG-psbA

LSC · 92-532 · 441 bp

Back to Quercus

Pi 0.0314
MarkerSeek score 67.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
441
variable_sites
92
pi
0.0314
unique_haplotype_count
64
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 505-589 bp 1.0 85.5
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 506-590 bp 1.0 85.5
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 519-603 bp 1.0 85.5
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCGTGCTAACCTTGGTATGG 50.0 50.0 57.9 57.3 520-604 bp 1.0 85.5
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 518-602 bp 1.0 85.4