Hotspot

rpl16

LSC · 84954-86361 · 1408 bp

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Pi 0.00295
MarkerSeek score 39.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1408
variable_sites
15
pi
0.00295
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCCCAAAAACCCCTTTCGTT TGGCAAACTCTTTCATTCTT 45.0 35.0 57.8 53.3 1519-1601 bp 1.0 43.4
rpl16_p2 TCCCAAAAACCCCTTTCGTT TGGCAAACTCTTTCATTCTTT 45.0 33.3 57.8 54.2 1519-1601 bp 1.0 42.6
rpl16_p3 ATCCCAAAAACCCCTTTCGT TGGCAAACTCTTTCATTCTT 45.0 35.0 57.6 53.3 1520-1602 bp 1.0 42.5
rpl16_p4 TCCCAAAAACCCCTTTCGTT ATGGCAAACTCTTTCATTCT 45.0 35.0 57.8 53.0 1520-1602 bp 1.0 42.5
rpl16_p5 TCCCAAAAACCCCTTTCGTT TGGCAAACTCTTTCATTCTTTT 45.0 31.8 57.8 55.0 1519-1601 bp 1.0 42.5