Hotspot

ndhF-rpl32

SSC · 116579-117639 · 1061 bp

Back to Pyrus

Pi 0.00605
MarkerSeek score 68.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1061
variable_sites
32
pi
0.00605
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 40.0 45.0 57.0 57.8 1225-1317 bp 1.0 76.3
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 40.0 45.0 57.0 57.8 1223-1315 bp 1.0 75.9
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 40.0 55.0 57.0 58.3 1212-1304 bp 1.0 75.0
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 40.0 45.0 57.0 57.6 1222-1314 bp 1.0 74.1
ndhF-rpl32_p5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 38.1 45.0 57.7 57.8 1226-1318 bp 1.0 74.1