Hotspot

petG-MW450_pgt022

LSC · 69680-69803 · 124 bp

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Pi 0.00926
MarkerSeek score 54.10
Primer available yes
Label petG-MW450_pgt022

Feature Metadata

label_name
petG-MW450_pgt022
feature_type
igs
region
LSC
length_bp
124
variable_sites
14
pi
0.00926
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-MW450_pgt022_p1 ACAGACGTGGTGATCAGTTG TTCAAATCCTACAGGGCGTG 50.0 50.0 57.8 57.9 173 bp 1.0 87.1
petG-MW450_pgt022_p2 ACAGACGTGGTGATCAGTTG GTTCAAATCCTACAGGGCGT 50.0 50.0 57.8 57.9 174 bp 1.0 87.1
petG-MW450_pgt022_p3 ACAGACGTGGTGATCAGTTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 192 bp 1.0 86.4
petG-MW450_pgt022_p4 ACAGACGTGGTGATCAGTTG AGAACGTGGGTCTCCAAAAC 50.0 50.0 57.8 58.0 206 bp 1.0 86.2
petG-MW450_pgt022_p5 ACAGACGTGGTGATCAGTTG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.8 58.0 228 bp 1.0 85.7