Hotspot

accD-psaI

LSC · 62246-62828 · 583 bp

Back to Pyrus

Pi 0.00213
MarkerSeek score 48.90
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
583
variable_sites
15
pi
0.00213
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGGGCGTTCTGAGTGAGTTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.4 58.0 715-1123 bp 0.967742 80.9
accD-psaI_p2 AGGGCGTTCTGAGTGAGTTA AATGGAGGGGAGACTGTTGA 50.0 50.0 58.4 58.0 672-1080 bp 0.967742 80.8
accD-psaI_p3 AGGGCGTTCTGAGTGAGTTA TTGCCGGAAATACTAAGCCC 50.0 50.0 58.4 58.0 706-1114 bp 0.967742 80.8
accD-psaI_p4 AGGGCGTTCTGAGTGAGTTA GAGGGGAGACTGTTGAGAGT 50.0 55.0 58.4 58.1 668-1076 bp 0.967742 80.7
accD-psaI_p5 AGGGCGTTCTGAGTGAGTTA GCACAAAAATGGAGGGGAGA 50.0 50.0 58.4 58.1 679-1087 bp 0.967742 80.6