Hotspot

ndhC-MW450_pgt021

LSC · 54251-55753 · 1503 bp

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Pi 0.0097
MarkerSeek score 64.00
Primer available yes
Label ndhC-MW450_pgt021

Feature Metadata

label_name
ndhC-MW450_pgt021
feature_type
igs
region
LSC
length_bp
1503
variable_sites
55
pi
0.0097
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-MW450_pgt021_p1 GGGGCTAAAACTCCGGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 1399-1721 bp 1.0 82.3
ndhC-MW450_pgt021_p2 CTGGTCCTTTGCTAATCGGG CCCGCCGTATGAAAGCAATA 55.0 50.0 58.3 58.4 1416-1738 bp 1.0 80.7
ndhC-MW450_pgt021_p3 CCTTTGCTAATCGGGGCTAA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.7 58.4 1411-1733 bp 1.0 80.6
ndhC-MW450_pgt021_p4 GGGGCTAAAACTCCGGAAAT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.9 57.3 1398-1720 bp 1.0 80.3
ndhC-MW450_pgt021_p5 GGGGCTAAAACTCCGGAAAT TTTGAACCAATGACTCCCGC 50.0 50.0 57.9 58.8 1414-1736 bp 1.0 79.5