Hotspot

psaA-pafI

LSC · 44843-45549 · 707 bp

Back to Pyrus

Pi 0.00115
MarkerSeek score 48.90
Primer available yes
Label psaA-pafI

Feature Metadata

label_name
psaA-pafI
feature_type
igs
region
LSC
length_bp
707
variable_sites
8
pi
0.00115
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-pafI_p1 GCCCATTCCTCGAAAGAAGT TGGAAACAAGCAATAGCGCT 50.0 45.0 57.9 58.5 862-912 bp 1.0 77.6
psaA-pafI_p2 GCCCATTCCTCGAAAGAAGT CAATAGCGCTTACTCCTGGT 50.0 50.0 57.9 57.4 852-902 bp 1.0 76.5
psaA-pafI_p3 GCCCATTCCTCGAAAGAAGT GGAGGCTTGGTTCGATCAAG 50.0 55.0 57.9 58.6 893-943 bp 1.0 76.2
psaA-pafI_p4 GCCCATTCCTCGAAAGAAGT GCAATAGCGCTTACTCCTGG 50.0 55.0 57.9 58.8 853-903 bp 1.0 75.1
psaA-pafI_p5 TTGAGAAATGACCCGGTCTG TGGAAACAAGCAATAGCGCT 50.0 45.0 57.5 58.5 882-932 bp 1.0 74.6