Hotspot

MW450_pgt012-MW450_pgt013

LSC · 39109-39465 · 357 bp

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Pi 0.00321
MarkerSeek score 50.00
Primer available yes
Label MW450_pgt012-MW450_pgt013

Feature Metadata

label_name
MW450_pgt012-MW450_pgt013
feature_type
igs
region
LSC
length_bp
357
variable_sites
8
pi
0.00321
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MW450_pgt012-MW450_pgt013_p1 TTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 483-574 bp 1.0 75.7
MW450_pgt012-MW450_pgt013_p2 AGTCTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.8 57.8 456-547 bp 1.0 74.2
MW450_pgt012-MW450_pgt013_p3 TCCGGCACATCATTATGGAT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 56.7 57.8 482-573 bp 1.0 73.8
MW450_pgt012-MW450_pgt013_p4 TTTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 42.9 50.0 57.7 57.8 484-575 bp 1.0 73.5
MW450_pgt012-MW450_pgt013_p5 TCCGGCACATCATTATGGATT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 57.5 57.8 482-573 bp 1.0 71.8