Hotspot

MW450_pgt011-psbD

LSC · 34372-35781 · 1410 bp

Back to Pyrus

Pi 0.00136
MarkerSeek score 53.40
Primer available yes
Label MW450_pgt011-psbD

Feature Metadata

label_name
MW450_pgt011-psbD
feature_type
igs
region
LSC
length_bp
1410
variable_sites
9
pi
0.00136
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MW450_pgt011-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1575-1713 bp 1.0 82.9
MW450_pgt011-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1578-1716 bp 1.0 82.7
MW450_pgt011-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1577-1715 bp 1.0 82.7
MW450_pgt011-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1565-1703 bp 1.0 82.7
MW450_pgt011-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1580-1718 bp 1.0 82.5