Hotspot

psbM-MW450_pgt008

LSC · 31813-33040 · 1228 bp

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Pi 0.00286
MarkerSeek score 50.20
Primer available yes
Label psbM-MW450_pgt008

Feature Metadata

label_name
psbM-MW450_pgt008
feature_type
igs
region
LSC
length_bp
1228
variable_sites
19
pi
0.00286
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-MW450_pgt008_p1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 1391-1418 bp 1.0 74.2
psbM-MW450_pgt008_p2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1393-1420 bp 1.0 72.4
psbM-MW450_pgt008_p3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1392-1419 bp 1.0 72.4
psbM-MW450_pgt008_p4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 57.6 57.0 1392-1419 bp 1.0 70.0
psbM-MW450_pgt008_p5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.1 58.5 1392-1419 bp 1.0 69.3