Hotspot

petN-psbM

LSC · 30492-31707 · 1216 bp

Back to Pyrus

Pi 0.00326
MarkerSeek score 48.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1216
variable_sites
16
pi
0.00326
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.98
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 50.0 45.0 58.0 57.6 1299-1324 bp 1.0 78.7
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1242-1267 bp 1.0 78.1
petN-psbM_p3 GTATGGGGAAGGAGTGGACT AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1241-1266 bp 1.0 78.1
petN-psbM_p4 TATGGGGAAGGAGTGGACTC AAAACAGTCAGCCAAGGTGA 55.0 45.0 57.8 57.6 1240-1265 bp 1.0 77.6
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG AAAACAGTCAGCCAAGGTGA 60.0 45.0 58.2 57.6 1237-1262 bp 1.0 77.3