Hotspot

rpoB-MW450_pgt007

LSC · 28183-29397 · 1215 bp

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Pi 0.00288
MarkerSeek score 43.80
Primer available yes
Label rpoB-MW450_pgt007

Feature Metadata

label_name
rpoB-MW450_pgt007
feature_type
igs
region
LSC
length_bp
1215
variable_sites
15
pi
0.00288
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-MW450_pgt007_p1 TAAGCCCTGCTCAATGAACC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1358-1373 bp 1.0 79.9
rpoB-MW450_pgt007_p2 TAAGCCCTGCTCAATGAACC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1340-1355 bp 1.0 77.7
rpoB-MW450_pgt007_p3 TATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1292-1307 bp 1.0 77.4
rpoB-MW450_pgt007_p4 CTCTGTTAAGCCCTGCTCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1364-1379 bp 1.0 77.1
rpoB-MW450_pgt007_p5 GTTCCTCTGTTAAGCCCTGC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.5 58.1 1368-1383 bp 1.0 76.5