Hotspot

MW450_pgt006-atpA

LSC · 10321-11157 · 837 bp

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Pi 0.0137
MarkerSeek score 61.90
Primer available yes
Label MW450_pgt006-atpA

Feature Metadata

label_name
MW450_pgt006-atpA
feature_type
igs
region
LSC
length_bp
837
variable_sites
68
pi
0.0137
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.74
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MW450_pgt006-atpA_p1 TGGAATGAAAAGCGTCCATTG AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 531-1006 bp 1.0 75.1
MW450_pgt006-atpA_p2 GGAATGAAAAGCGTCCATTGT AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 530-1005 bp 1.0 75.1
MW450_pgt006-atpA_p3 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 47.6 50.0 57.3 58.4 470-945 bp 1.0 74.7
MW450_pgt006-atpA_p4 TGGATAGGACAGAGGTCTTCT AGACATTTACCGACGAAGCG 47.6 50.0 56.9 58.4 505-980 bp 1.0 71.5
MW450_pgt006-atpA_p5 TGGAATGAAAAGCGTCCATT AGACATTTACCGACGAAGCG 40.0 50.0 55.9 58.4 531-1006 bp 1.0 71.1