Hotspot

matK

LSC · 220-1725 · 1506 bp

Back to Pyrrosia

Pi 0.0643

MarkerSeek score 75.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 272
pi: 0.0643
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AAGCAAGCCGTTGATAAGCT	GTGAAACCCGAGCTAGGAAG	45.0	55.0	58.2	58.0	1797 bp	0.2	46.5
matK_p2	AAGCAAGCCGTTGATAAGCT	AGGTAGCTCAATCAACAGCG	45.0	50.0	58.2	58.0	2310 bp	0.2	46.5
matK_p3	GAAGCAAGCCGTTGATAAGC	GTGAAACCCGAGCTAGGAAG	50.0	55.0	57.8	58.0	1798 bp	0.2	46.5
matK_p4	GCAAGCCGTTGATAAGCTTC	GTGAAACCCGAGCTAGGAAG	50.0	55.0	57.8	58.0	1795 bp	0.2	46.5
matK_p5	GCAAGCCGTTGATAAGCTTC	AGGTAGCTCAATCAACAGCG	50.0	50.0	57.8	58.0	2308 bp	0.2	46.4