Hotspot

psbE-petL

LSC · 60706-61478 · 773 bp

Back to Pyrrosia

Pi 0.0699

MarkerSeek score 82.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 150
pi: 0.0699
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	AGGCGAAGTAACTTAATAGAGTGA	55.0	37.5	58.1	57.2	800-827 bp	1.0	53.4
psbE-petL_p2	GCGTTCTCCTGTATTCCCAG	GGCGAAGTAACTTAATAGAGTGA	55.0	39.1	58.1	55.9	799-826 bp	1.0	52.0
psbE-petL_p3	GCGTTCTCCTGTATTCCCAG	AGGCGAAGTAACTTAATAGAGTG	55.0	39.1	58.1	55.9	800-827 bp	1.0	52.0
psbE-petL_p4	GCGTTCTCCTGTATTCCCAG	AAGGCGAAGTAACTTAATAGAGTGA	55.0	36.0	58.1	57.8	801-828 bp	1.0	52.0
psbE-petL_p5	GCGTTCTCCTGTATTCCCAG	AGGCGAAGTAACTTAATAGAGTGAT	55.0	36.0	58.1	57.7	800-827 bp	1.0	52.0