Hotspot

petA-psbJ

LSC · 58144-59942 · 1799 bp

Back to Pyrrosia

Pi 0.104

MarkerSeek score 75.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1799
variable_sites: 425
pi: 0.104
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGGGAAAGGGTGGATACGAA	GTAAGGAGGTGGCACAGATG	50.0	55.0	58.0	58.0	2238-2932 bp	0.6	73.1
petA-psbJ_p2	AGGGAAAGGGTGGATACGAA	TAAGGAGGTGGCACAGATGA	50.0	50.0	58.0	58.0	2237-2931 bp	0.6	72.9
petA-psbJ_p3	CAAACGGAAAAAGAGGGGGA	GTAAGGAGGTGGCACAGATG	50.0	55.0	58.0	58.0	2610-2619 bp	0.2	48.0
petA-psbJ_p4	CAAACGGAAAAAGAGGGGGA	TAAGGAGGTGGCACAGATGA	50.0	50.0	58.0	58.0	2609-2618 bp	0.2	47.8
petA-psbJ_p5	CAAACGGAAAAAGAGGGGGA	TCATGTTTCGAGTCGGAACC	50.0	50.0	58.0	57.9	2656-2665 bp	0.2	47.6