Hotspot

rps4-trnL-UAA

LSC · 43329-43951 · 623 bp

Back to Pyrrosia

Pi 0.0649
MarkerSeek score 75.80
Primer available yes
Label rps4-trnL-UAA

Feature Metadata

label_name
rps4-trnL-UAA
feature_type
igs
region
LSC
length_bp
623
variable_sites
113
pi
0.0649
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnL-UAA_p1 GTCCTCGGTAGCGAGACAT CTACCATTCCGCCATATCCC 57.9 55.0 58.6 57.6 654-664 bp 1.0 74.1
rps4-trnL-UAA_p2 GTCCTCGGTAGCGAGACAT CGTCTACCATTCCGCCATAT 57.9 50.0 58.6 57.3 657-667 bp 1.0 71.4
rps4-trnL-UAA_p3 GTCCTCGGTAGCGAGACA CTACCATTCCGCCATATCCC 61.1 55.0 58.1 57.6 654-664 bp 1.0 70.0
rps4-trnL-UAA_p4 GTCCTCGGTAGCGAGACAT CAATCTGGTTGAGTCCGCTG 57.9 55.0 58.6 58.9 677-687 bp 1.0 69.9
rps4-trnL-UAA_p5 GTCCTCGGTAGCGAGACAT TACCATTCCGCCATATCCCC 57.9 55.0 58.6 59.0 653-663 bp 1.0 69.2