Hotspot

psbM-petN

LSC · 26853-27814 · 962 bp

Back to Pyrrosia

Pi 0.0762

MarkerSeek score 77.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 197
pi: 0.0762
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GGCCGCGCAGAATAACTAG	TTGTAGTTTGGGGACGAAGC	57.9	50.0	58.8	58.1	979-1052 bp	1.0	76.9
psbM-petN_p2	GGCCGCGCAGAATAACTAG	CACTTGTAGTTTGGGGACGA	57.9	50.0	58.8	57.5	982-1055 bp	1.0	73.5
psbM-petN_p3	GGCCGCGCAGAATAACTAG	TCACTTGTAGTTTGGGGACG	57.9	50.0	58.8	57.5	983-1056 bp	1.0	73.5
psbM-petN_p4	GGCCGCGCAGAATAACTA	TTGTAGTTTGGGGACGAAGC	55.6	50.0	57.6	58.1	979-1052 bp	1.0	71.6
psbM-petN_p5	GGCCGCGCAGAATAACTAG	GCCTTGACGGTGATTTCCA	57.9	52.6	58.8	58.1	1013-1086 bp	1.0	69.3