Hotspot

rpoB-trnD-GUC

LSC · 25335-26007 · 673 bp

Back to Pyrrosia

Pi 0.079

MarkerSeek score 83.50

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 154
pi: 0.079
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	GGTGCTCTAACCGATTGAACT	55.0	47.6	58.0	58.0	723-743 bp	1.0	81.7
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	GGTGCTCTAACCGATTGAACT	55.0	47.6	58.0	58.0	724-744 bp	1.0	81.7
rpoB-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	766-786 bp	1.0	81.4
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	767-787 bp	1.0	81.4
rpoB-trnD-GUC_p5	AGTTCTGGTAGTACGGGCAT	GGTGCTCTAACCGATTGAACT	50.0	47.6	58.1	58.0	721-741 bp	1.0	80.7