Hotspot

trnS-GCU-ycf12

LSC · 6657-7203 · 547 bp

Back to Pyrrosia

Pi 0.0685

MarkerSeek score 80.50

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 106
pi: 0.0685
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	CCGACGCTTTAAACCTCTCA	TCGGCTAGATGTTCCAAACG	50.0	50.0	57.9	58.0	809-810 bp	0.2	47.5
trnS-GCU-ycf12_p2	GGGAGAGAGAGGGATTCGAA	TCGGCTAGATGTTCCAAACG	55.0	50.0	57.9	58.0	866-867 bp	0.2	47.5
trnS-GCU-ycf12_p3	AAGGGAGAGAGAGGGATTCG	TCGGCTAGATGTTCCAAACG	55.0	50.0	57.9	58.0	868-869 bp	0.2	47.4
trnS-GCU-ycf12_p4	TGCCTCAATTGTCTCGTCAG	TCGGCTAGATGTTCCAAACG	50.0	50.0	57.9	58.0	929-930 bp	0.2	47.4
trnS-GCU-ycf12_p5	CCGACGCTTTAAACCTCTCA	CGAAGTACAAGAGTGACGGG	50.0	55.0	57.9	58.0	1025-1026 bp	0.2	47.3