Hotspot

ycf1

SSC · 126460-132075 · 5616 bp

Back to Pyrenaria

Pi 0.00526

MarkerSeek score 43.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5616
variable_sites: 99
pi: 0.00526
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCGATCTTGATCCAGGCCT	TTGGTTCTCCCCAAAAACGG	50.0	50.0	58.3	58.6	1529 bp	1.0	74.9
ycf1_p2	ATCGATCTTGATCCAGGCCT	CCAAAAACGGCCTTCCTTTT	50.0	45.0	58.3	57.4	1519 bp	1.0	74.8
ycf1_p3	ATCGATCTTGATCCAGGCCT	CTTCCTTTTGGTTCTCCCCA	50.0	50.0	58.3	57.3	1536 bp	1.0	74.3
ycf1_p4	ATCGATCTTGATCCAGGCCT	ACTTCCTTTTGGTTCTCCCC	50.0	50.0	58.3	57.3	1537 bp	1.0	74.3
ycf1_p5	ATCGATCTTGATCCAGGCCT	CGGTTTGGGGAATGAAAGCT	50.0	50.0	58.3	58.7	1559 bp	1.0	73.7