Hotspot

rps3

LSC · 85289-85945 · 657 bp

Back to Pyrenaria

Pi 0.00548

MarkerSeek score 36.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 10
pi: 0.00548
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGTTCCATTACTGGGGTAGA	TATGGATAGTAGCGGGGGAG	45.0	55.0	55.1	57.5	721 bp	0.909091	50.2
rps3_p2	TGTTCCATTACTGGGGTAGAA	TATGGATAGTAGCGGGGGAG	42.9	55.0	56.0	57.5	721 bp	0.909091	48.9
rps3_p3	TTGTTCCATTACTGGGGTAGA	TATGGATAGTAGCGGGGGAG	42.9	55.0	56.0	57.5	722 bp	0.909091	48.9
rps3_p4	ACTGGGGTAGAAAGAAAAGACA	TATGGATAGTAGCGGGGGAG	40.9	55.0	56.9	57.5	712 bp	0.909091	48.7
rps3_p5	TGTTCCATTACTGGGGTAGAAAG	TATGGATAGTAGCGGGGGAG	43.5	55.0	57.8	57.5	721 bp	0.909091	47.8