Hotspot

rpl16

LSC · 83735-85160 · 1426 bp

Back to Pyrenaria

Pi 0.00388

MarkerSeek score 43.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1426
variable_sites: 17
pi: 0.00388
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTTCTTTCAATGCAAGGGGC	TCGATTGACCATTTGATATAATTGCT	50.0	30.8	57.9	57.6	1535-1550 bp	1.0	41.8
rpl16_p2	GTTCTTTCAATGCAAGGGGC	TCGATTGACCATTTGATATAATTGC	50.0	32.0	57.9	56.4	1535-1550 bp	1.0	41.8
rpl16_p3	GTTCTTTCAATGCAAGGGGC	GGGTTGAATAAAAATTCGATTGACC	50.0	36.0	57.9	57.5	1550-1565 bp	1.0	41.7
rpl16_p4	GTTCTTTCAATGCAAGGGGC	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.9	56.9	1549-1564 bp	1.0	41.7
rpl16_p5	GTTCTTTCAATGCAAGGGGC	AGGGTTGAATAAAAATTCGATTGAC	50.0	32.0	57.9	56.6	1551-1566 bp	1.0	41.7