Hotspot

ndhF

SSC · 112757-115003 · 2247 bp

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Pi 0.0063

MarkerSeek score 41.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 44
pi: 0.0063
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	TGACCAATTGTGACTTCGTGA	50.0	42.9	58.0	57.8	2684-2767 bp	1.0	72.1
ndhF_p2	GCAGAATACCGTCACCCATT	TGACCAATTGTGACTTCGTGA	50.0	42.9	58.0	57.8	2773-2856 bp	1.0	71.9
ndhF_p3	ACAGGAACAAGAGGGATCCA	TGACCAATTGTGACTTCGTGA	50.0	42.9	58.0	57.8	2670-2753 bp	1.0	71.9
ndhF_p4	AAGAGGGATCCACCGAAGAA	TGACCAATTGTGACTTCGTGA	50.0	42.9	58.0	57.8	2662-2745 bp	1.0	71.9
ndhF_p5	AGGGATCCACCGAAGAAGAT	TGACCAATTGTGACTTCGTGA	50.0	42.9	57.8	57.8	2659-2742 bp	1.0	70.8