Hotspot

matK

LSC · 2047-3546 · 1500 bp

Back to Pyrenaria

Pi 0.00436

MarkerSeek score 52.90

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 18
pi: 0.00436
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GGGATAATTTCGATTTACACGGT	TGTTTTGACCGTATCGCACT	39.1	45.0	57.0	57.8	1610-1616 bp	1.0	47.7
matK_p2	GGGATAATTTCGATTTACACGGTC	TGTTTTGACCGTATCGCACT	41.7	45.0	58.0	57.8	1610-1616 bp	1.0	47.5
matK_p3	AGGGATAATTTCGATTTACACGG	TGTTTTGACCGTATCGCACT	39.1	45.0	56.7	57.8	1611-1617 bp	1.0	45.6
matK_p4	AGGGATAATTTCGATTTACACGGT	TGTTTTGACCGTATCGCACT	37.5	45.0	58.3	57.8	1611-1617 bp	1.0	45.1
matK_p5	GGGATAATTTCGATTTACACGGT	CCTTGTTTTGACCGTATCGC	39.1	50.0	57.0	57.5	1613-1619 bp	1.0	44.8