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rps15-ycf1

SSC · 126070-126459 · 390 bp

Back to Pyrenaria

Pi 0.00578

MarkerSeek score 26.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 5
pi: 0.00578
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AACCCCTTCGTCTGTCTGTA	45.0	50.0	58.1	58.0	1349-1366 bp	1.0	81.7
rps15-ycf1_p2	AACACCATGCATTGATGGGT	ACCCCTTCGTCTGTCTGTAA	45.0	50.0	58.1	58.0	1348-1365 bp	1.0	81.7
rps15-ycf1_p3	CTTGTAGCTGGTGCAGTCAT	ACCCCTTCGTCTGTCTGTAA	50.0	50.0	57.9	58.0	1293-1310 bp	1.0	81.5
rps15-ycf1_p4	TTCCCAATTGTTCTGGTGCA	AACCCCTTCGTCTGTCTGTA	45.0	50.0	57.9	58.0	1545-1562 bp	1.0	81.3
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	ACCCCTTCGTCTGTCTGTAA	45.0	50.0	57.9	58.0	1544-1561 bp	1.0	81.3