Hotspot

rpl32-trnL-UAG

SSC · 115851-116773 · 923 bp

Back to Pyrenaria

Pi 0.00719
MarkerSeek score 52.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
923
variable_sites
22
pi
0.00719
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTCAACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 999-1016 bp 1.0 73.8
rpl32-trnL-UAG_p2 AGGGAAATCTCTTTCAACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 1000-1017 bp 1.0 73.7
rpl32-trnL-UAG_p3 GGGAAATCTCTTTCAACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1006-1023 bp 0.909091 70.6
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTCAACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1007-1024 bp 0.909091 70.6
rpl32-trnL-UAG_p5 AGGATATTGGGCAGCTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 56.9 57.9 1040-1057 bp 0.909091 70.0