Hotspot

ndhF-rpl32

SSC · 115004-115688 · 685 bp

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Pi 0.00874

MarkerSeek score 47.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 16
pi: 0.00874
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGCCCACATACGACGAA	TAGATTGGGTTGAAAGCGGG	45.0	50.0	57.8	57.9	1466-1487 bp	1.0	80.9
ndhF-rpl32_p2	AAAGCCCACATACGACGAAG	TAGATTGGGTTGAAAGCGGG	50.0	50.0	58.3	57.9	1465-1486 bp	1.0	80.1
ndhF-rpl32_p3	GAAAAGCCCACATACGACGA	TAGATTGGGTTGAAAGCGGG	50.0	50.0	58.3	57.9	1467-1488 bp	1.0	80.1
ndhF-rpl32_p4	AAAAGCCCACATACGACGAA	ATTGGGTTGAAAGCGGGAAA	45.0	45.0	57.8	58.3	1463-1484 bp	1.0	79.6
ndhF-rpl32_p5	AAAAGCCCACATACGACGAA	TTGGGTTGAAAGCGGGAAAT	45.0	45.0	57.8	58.3	1462-1483 bp	1.0	79.6