Hotspot

accD-psaI

LSC · 60695-61398 · 704 bp

Back to Pyrenaria

Pi 0.00495

MarkerSeek score 53.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 10
pi: 0.00495
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	798-824 bp	1.0	77.2
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	804-830 bp	1.0	76.6
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	798-824 bp	1.0	74.8
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	796-822 bp	1.0	74.7
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	809-835 bp	1.0	74.5