Hotspot

trnS-GGA-rps4

LSC · 47382-47673 · 292 bp

Back to Pyrenaria

Pi 0.00928
MarkerSeek score 51.50
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
292
variable_sites
7
pi
0.00928
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 ATGTAGGAGAGATGGCCGAG AGTAAATGGGTCAGTTTGAA 55.0 35.0 58.4 52.3 445-450 bp 1.0 42.1
trnS-GGA-rps4_p2 ATGTAGGAGAGATGGCCGAG AGTAAATGGGTCAGTTTGAAA 55.0 33.3 58.4 53.2 445-450 bp 1.0 42.1
trnS-GGA-rps4_p3 ATGTAGGAGAGATGGCCGAG AGTAAATGGGTCAGTTTGAAAA 55.0 31.8 58.4 54.1 445-450 bp 1.0 42.1
trnS-GGA-rps4_p4 TTCACTCGGTTTTTGGGTCA AGTAAATGGGTCAGTTTGAA 45.0 35.0 57.6 52.3 473-478 bp 1.0 42.0
trnS-GGA-rps4_p5 TTCACTCGGTTTTTGGGTCA AGTAAATGGGTCAGTTTGAAA 45.0 33.3 57.6 53.2 473-478 bp 1.0 42.0