Hotspot

rpoB-trnC-GCA

LSC · 27867-29103 · 1237 bp

Back to Pyrenaria

Pi 0.00417

MarkerSeek score 53.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 17
pi: 0.00417
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCATTTCCATCCCGTAGCA	CCAGATTTGAACCGGGGAAA	47.6	50.0	58.1	58.1	1311-1322 bp	1.0	72.4
rpoB-trnC-GCA_p2	TTCATTTCCATCCCGTAGCA	CCAGATTTGAACCGGGGAAA	45.0	50.0	56.9	58.1	1310-1321 bp	1.0	72.3
rpoB-trnC-GCA_p3	TTCCTTCATTTCCATCCCGT	CCAGATTTGAACCGGGGAAA	45.0	50.0	56.8	58.1	1314-1325 bp	1.0	71.5
rpoB-trnC-GCA_p4	AGTTCTTCCGTCAAGCTTTGA	CCAGATTTGAACCGGGGAAA	42.9	50.0	57.8	58.1	1393-1404 bp	1.0	71.4
rpoB-trnC-GCA_p5	TCATTTCCATCCCGTAGCAT	CCAGATTTGAACCGGGGAAA	45.0	50.0	56.7	58.1	1309-1320 bp	1.0	70.5