Hotspot

ycf3

Genome · 47562-49519 · 1958 bp

Back to Pyracantha

Pi 0

MarkerSeek score 57.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 1958
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATCCTCGATAGGGACAACA	GTGGGATCATTTTCTCACGA	47.6	45.0	58.0	55.5	2109-2110 bp	1.0	51.6
ycf3_p2	TCCCAGAGAAATGATCCTCGA	GTGGGATCATTTTCTCACGA	47.6	45.0	58.0	55.5	2120-2121 bp	1.0	51.5
ycf3_p3	TGATCCTCGATAGGGACAACA	GTGGGATCATTTTCTCACGAA	47.6	42.9	58.0	56.3	2109-2110 bp	1.0	49.9
ycf3_p4	TCCCAGAGAAATGATCCTCGA	GTGGGATCATTTTCTCACGAA	47.6	42.9	58.0	56.3	2120-2121 bp	1.0	49.8
ycf3_p5	CCTCGATAGGGACAACAAACA	GTGGGATCATTTTCTCACGA	47.6	45.0	57.7	55.5	2105-2106 bp	1.0	49.1