Hotspot

ndhF

Genome · 116772-119027 · 2256 bp

Back to Pyracantha

Pi 0.000443

MarkerSeek score 57.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2256
variable_sites: 1
pi: 0.000443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCTGGATGGGAATCAAGAACA	GAACCGGTGACTCAGAAACA	42.9	50.0	56.6	57.8	2351 bp	1.0	59.1
ndhF_p2	TCTGGATGGGAATCAAGAACAA	GAACCGGTGACTCAGAAACA	40.9	50.0	57.3	57.8	2351 bp	1.0	56.9
ndhF_p3	TGGATGGGAATCAAGAACAAGA	GAACCGGTGACTCAGAAACA	40.9	50.0	57.3	57.8	2349 bp	1.0	56.9
ndhF_p4	TCTGGATGGGAATCAAGAACA	AGAACCGGTGACTCAGAAAC	42.9	50.0	56.6	57.5	2352 bp	1.0	56.8
ndhF_p5	TCTGGATGGGAATCAAGAACA	AACCGGTGACTCAGAAACAA	42.9	45.0	56.6	57.3	2350 bp	1.0	55.4